7 31 2014 oyster bay repro check

Kamilche shores, wa

Hi 60s to low 80s sunny

Participants: Sean Bennett and Jake heare

Performed anesthesia sop with insulation on treatment tub and ice blocks on recovery tubs.  Found very few brooders again. I think it is a stack effect due to the unholy amounts of mussels set on the trays and oysters. Tried to clean them off but too many. Mussels probably consuming all sperm in water column.

Numbers as follow

Temps in c
Pretreatment
Initial.       17
45.          18
1.5.           22

Treatment
Initial.      15
45.         14
1.5.         15
2.25.       16

Recovery
Initial.     18
45.         20
1.5.         21

Salinity in ppt
Pretreatment.      26
Treatment.         60
Recovery.           27

Brood collection

1N5-8
Brood.      1
Gaping.     50
Dead.        0
Closed.      0

Brooders
#   size.   Sick
1.     30.     W.    Partial sample due to spillage.

1S13-16
Brood.    0
Gaping.   70
Dead.      0
Closed.    2

1H1-4
Brood.      1
Gaping.     59
Dead.        2
Closed.     8

Brooders
#     size.   Sick
1.       32.      Grey

7 30 2014 Manchester Repro Check

Manchester WA

Hi 60's to low 80's

Performed the anesthesia SOP with insulation on the treatment tub and water addition to the pretreatment and recovery tubs. Found one brooder in Southern and Dabob populations each. Also talked to Stuart at PSRF about spawning groups, he said they spawn Olys in groups of twenty.

Numbers as Follow

Temps in C

Pretreatment

Initial    11
45        12
1.5       14

Treatment
Initial    13
45        13
1.5       13
2.25     13

Recovery
Initial     13
45         15
1.5        17

Salinity in ppt

Pretreatment   28
Treatment       60
Recovery        27

Brood Collection

4S13-16
Brood   1
Gaping  69
Dead     10
Closed   10

Brooder
#   Size  Sick
1    24    W   *Sample of poor quality due to screen failure

4N1-4
Brood     0
Gaping    45
Dead      12
Closed    7

4H9-12
Brood     1
Gaping    45
Dead       9
Closed    11

Brooder
#     size     sick
1     20       w

Pics have brooders between tube label and caliper. Closed oysters on the same tile. Open oysters on the other three tiles. Some oysters on closed/brooder tiles are open and vice versa due to the fact of the guys glued to the tiles.

7 28 2014 Temp Data Manipulation

I attempted to manipulate the temp data today. I'm running into the same sed error on the windows computers that jammed me up back in the winter. I can edit pretty much anything and produce an out file but for some reason if I try to eliminate the quotation marks using sed so that I can have clean column heading, sed will not produce an outfile or even change the file its working on. It shows the edit in the notebook but then doesn't create the actual change in the file. Does anyone know how to solve this other than bouncing back and forth between my linux computer and desktop.

See attached notebook

7 25 2014 Fidalgo repro check

Anacortes wa

Mid 60s to mid 70s sunny

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Treatment less effective than normal but still had an acceptable amount of Gaping oysters. Found many grey sick animals in the south pop.

Numbers as follow :

Temps in c
Pretreatment
Initial.          9
45.             11
1.5.             11

Treatment.
Initial.        11
45.           11
1.5.            11
2.25.         11

Recovery
Initial.         11
45.            12
1.5.            14

Salinity in ppt
Pretreatment.      27
Treatment.          63
Recovery.           25

Brood collection
2N9-12
Brood.      0
Gaping.     55
Dead.        0
Closed.      45

2H1-4
Brood.       2
Gaping.      50
Dead.         0
Closed.      36

Brooders
#     size.   Sick
1.       20.      W
2.      22.       W

2S5-8
Brood.     6
Gaping.    72
Dead.       0
Closed.    25

Brooders
#     size.    Sick
1.       29.      Grey
2.      25.      Grey
3.      30.      Grey
4.      30.      W
5.      32.      W
6.      31.       Grey

Oyster below calipers closed. Oysters between calipers and label tube brooders.

7 24 2014. Oyster bay repro check

Oyster bay wa

High 60s to low 70s partly cloudy

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Brooding back to normal with most brooders in southern pop, and equal numbers between north and dabob. Also crab fresh cleaning salt water lines used their water for Pretreatment but not for recovery because pump was shut down.

Numbers as follow
Temps in c

Pretreatment
Initial.    15
45.        15
1.5.        15

Treatment
Initial.    12
45.        12
1.5.        12
2.25.     13

Recovery
Initial.    13
45.        13
1.5.        14

Brood collection

1S1-4
Brood.      8
Gaping.     75
Dead.        4
Closed.     7

Brooders
#    size.    Sick
1.      24.      W
2.     34.       W
3.     30.       W
4.     30.      W
5.     30.      W
6.     25.      W
7.     26.      W
8.     27.       W

1N9-12
Brood.       3
Gaping.     68
Dead.        0
Closed.      10

Brooders
#     size.     Sick
1.      33.        W
2.     28.        W
3.     30.        W

1H9-12
Brood.     4
Gaping.    72
Dead.       4
Closed.    6

Brooders
#      size.      Sick
1.        25.        W
2.       26.        W
3.       28.        W
4.       25.        W.    No sample.

Also pics are of whole tray. Brooders between tube and calipers or bag. Closed same time outside of brooders. Rest are gapers.

7 23 14 Manchester Repro Survey

Manchester WA

Hi 50's to mid 60's Rainy, overcast

Participants: Joseph Stevick and Jake Heare

Performed the anesthesia sop with insulation on the treatment tub and tarp on the pretreatment/recovery tubs. No ice blocks because of the cloudy cool conditions. Found a few brooders in the Southern pop and one in the Dabob pop. Very middle of the road spawning.

Numbers as follow

Temp in C

Pretreatment
initial          8
45 min       9
1.5 hr        8

Treatment
Initial         8
45 min       9
1.5 hr        8
2.25  hr     8

Recovery
Initial        8
45 min      8
1.5 hr       8

Salinity in ppt

Pretreatment    27
Treatment        70
Recovery         28

Brood collection

4H5-8
Brood   1
Gaping  67
dead      7
closed    9

brooders
#     size   sick
1     15     w

4S1-4
Brood   2
Gaping  59
Dead     1
closed    6

Brooders
#    size   sick
1    27     w
2    23     w

4N5-8
Brood    0
Gaping   60
Dead     10
Closed   9

Also took pictures of the entire population. In the pics, the tile with the caliper and label tube are the closed animals except for the animals that are between the tube and calipers which were brooders.

7 18 2014 fidalgo bay repro check

Anacortes, WA

Mid 60s to mid 70s

Participants: alicia Godersky and Jake heare

Performed anesthesia sop with insulation on treatment tub.  Found brooders in only the dabob pop. It seems that they spawn later in the season but in smaller number. We will see in the coming weeks. Also treatment was surprisingly ineffective with no discernable reason. Water was calm. Temps were stable. No movement while in tubs. Full treatment strength and time.  Hopefully it was a fluke.

Numbers as follow:

Temps in c
Pretreatment
Initial.     10
45.         11
1.5.         11

Treatment
Initial.   10
45.       10
1.5.       11
2.25.     11

Recovery
Initial.      10
45.          11
1.5.          12

Salinity in ppt
Pretreatment.       28
Treatment.           66
Recovery.            28

Brood collection

2S1-4
Brood.      0
Gaping.     67
Dead.        0
Closed.     30

2H9-12
Brood.      2
Gaping.    32
Dead.       0
Closed.     50

Brooders
#     size.     Sick
1.       19.        W
2.      20.       W

2N13-16
Brood.     0
Gaping.    68
Dead.       0
Closed.    40

7 17 2014 oyster bay repro

Oyster bay

High 60s to high 70s

Participants:  alicia Godersky and Jake heare

Performed anesthesia sop with insulation on treatment and tarp with ice blocks on recovery tubs. Very heavy mussel set on all trays and oysters. Possibly causing spawning issues. Only one population had brooders which is very different than in previous weeks. The one pop was the dabob pop with 5 brooders, one of which looked like aborted spawn.

Numbers as follow.

Temps in c
Pretreatment
Initial.   16
45.      18
1.5.       18

Treatment
Initial.     13
45.         14
1.5.         14
2.25.       14

Recovery
Initial.      16
45.          16
1.5.          17

Salinity in ppt
Pretreatment.       24
Treatment.           66
Recovery.            25

Brood collection
1S13-16.        
Brood.     0
Gaping.     75
Dead.       1
Closed.     2

1N5-8
Brood.      0
Gaping.     48
Dead.        1
Closed.      1

1H1-4
Brood.      5. 
Gaping.     68
Dead.        6
Closed.      1

Brooders
#     size.    Sick
1.       30.      W
2.       20.     W
3.       26.      W
4.       26.      W
5.       27.      W. Possibly abort spawn.

7 16 2014 Manchester Repro Check

Manchester WA

Low 70s to Mid 80s

Participants: Alicia Godersky and Jake Heare

Performed the anesthesia SOP with insulation and ice blocks on the treatment group and tarp over the recovery and pretreatment tubs. Finally found multiple brooders in one population. If you can guess the pop, you can consider yourself an Oly Whisperer. The brooding animals were also much smaller than expected. Half were less than 20 mm which is quite odd.  Also temps fluxed on the tubs on the dock even with replacing water, the combination of sun and no breeze just baked the docks.

Numbers as follow:

Temps in C
Pretreatment
Initial     10
45         12
1.5        13

Treatment
Initial      12
45          13
1.5         12
2.25       11

Recovery
Initial      12
45          14
1.5         15


Salinity in ppt
Pretreatment      26
Treatment          66
Recovery           25


Brood Collection
4S5-8
Brood    3
Gaping   59
Dead      12
Closed   12

Brooders
#     size     sick
1      22      w
2      16      w
3      20      w

4H13-16
Brood     1
Gaping    56
Dead       8
Closed    17

Brooders
#     size    sick
1      17     W

4N13-16
Brood     0
Gaping    61
Dead       6
Closed    8

7 11 2014 fidalgo repro check

Anacortes, wa

Low 70s to mid 80s

Performed the anesthesia sop with insulation and ice blocks on treatment and tarp cover on Pretreatment and recovery.  Found lots of brooders in southern pop and none in the other two pops. South sound oysters seem to be very attune to tidal changes. Also temps remained within range using the tarp cover. I think I should add a board or stand to keep the tarp higher over the tubs to keep it out of the water and allow for air flow to keep air temps lower. Also note to self, next week I absolutely have to refill my EtOH, 50 ml falcon tubes, and zipties. Also need a new water proof write in the rain pen as I have lost my only good one.

Numbers as follow :

Temps in c
Pretreatment
Initial.   16
45.         16
1.5.        17

Treatment
Initial.          14
45.                15
1.5.               14
2.25.             14

Recovery
Initial.         16
45.               16
1.5.              17

Salinity
Pretreatment.         20
Treatment.              68
Recovery.               23

Brood collection

2H5-8
Brood.     0
Gaping.   70
Dead.       0
Closed.    28

2S13-16
Brood.       11
Gaping.     81
Dead.         0
Closed.      11

Brooders
#    size.    Sick
1.    27.       W
2.    25.       W
3.    26.       W
4.    30.       W
5.    28.       W
6.    30.       W
7.    27.       W
8.    31.       W
9.    25.       W
10.  32.       W
11.  31.       W

2N1-4
Brood.      0
Gaping.    78
Dead.        0
Closed.     17

7 10 2014 oyster bay repro check

Klamiche shores, wa

Hi 60s to low 80s

Performed the anesthesia sop with insulation and ice blocks on treat. I also covered the Pretreatment and recovery tubs with a tarp to shade them from direct sun to help moderate the water temps. It worked well along with adding fresh seawater and leftover ice blocks, keeping the trays within 3 degrees of ambient. Also I broke the one day record for number of brooders. All pops had multiple brooders with the northern pop almost being equal to the southern pop in terms of numbers. Dabob pop also performed very well. I think this has to do with last week's neap tide and the much warmer than average air temps.

Numbers as follow.

Temps in c
Pretreatment
Initial.        16
45.              16
1.5.             16

Treatment
Initial.         14
45.               14
1.5.              13
2.25.            12

Recovery
Initial.          16
45.               17
1.5.               17

Salinity in ppt
Pretreatment.     25
Treatment.          65
Recovery.            24

Brood collection

1N9-12
Brood.      8
Gaping.    73
Dead.        3
Closed.     2

Brooders
#     size.     Sick
1.     30.        W
2.     22.        W  no sample, very few larvae
3.     39.        W
4.     35.        W
5.     31.        W
6.     33.        W
7.     28.        W
8.     32.        W  no sample tile oyster

1H9-12
Brood.       6
Gaping.     83
Dead.         3
Closed.      3

Brooders
#      size.     Sick
1.      27.        W
2.      27.        W.
3.      28.        W
4.      26.        W
5.      26.        W
6.      27.        W

1S1-4
Brood.      10
Gaping.    82
Dead.        0
Closed.     1

Brooders
#        size.       Sick
1.        22.          W
2.        22.          W
3.        25.          W
4.        28.          W
5.        27.          W
6.        27.          W
7.        25.          W
8.        24.          W
9.        30.          W
10.      30.          W.  No sample, tile oyster

7 9 2014 Manchester Repro Check

Manchester, WA

Hi 60s to Mid 80s, sunny

Participants: Joe Stevick and Jake Heare

Used the anesthesia SOP with insulation modification and ice blocks in both the treatment and recovery trays. Treatment stayed within 1 degree, while recovery trays stayed within 5 degrees. Found 1 brooder from the Dabob population which was pretty interesting considering that none of the other populations have been spawning.

Numbers as follow:

Temps in C

Pretreatment
Initial        11
45 min      13
1.5 hr       16


Treatment
Initial       11
45 min     12
1.5 hr      11
2.25 hr    10

Recovery
Initial       13
45 min     16
1.5 hr      17

Salinity in PPT

Pretreatment     26
Treatment        53
Recovery         27


Brood Collection
4N9-12
Brood       0
Gaping      45
Dead        12
Closed      2

4S9-12
Brood       0
Gaping      55
Dead        22
Closed      15

4H1-4
Brood       1
Gaping      60
Dead         5
Closed      21

Brooders
#       size     sick
1       21       w

7 7 2014 Running Tally of Brooders

Here's an updated running tally of brooders. It appears the numbers for fidalgo were a bit weaker than I thought this morning during lab meeting.

Total Brooders	Manchester	Oyster Bay	Fidalgo
N	1	15	3
H	1	8	1
S	1	41	17
1st brood	6/18/2014	5/29/2014	6/6/2014

7 4 2014 Fidalgo repro check

Anacortes wa

Mid 60s to low 70s

Participants: Jim cerkovnik and Jake heare

Performed anesthesia sop with insulation on treatment tub. Found brood Dr s in all three pops. Majority in southern pop, equal numbers in northern and dabob.

Numbers as follow:

Temps in c
Pretreatment
Initial.        9
45              11
1.5.            12

Treatment
Initial.        12
45.              12
1.5.             12
2.25.           12

Recovery
Initial.          13
45.                13
1.5.               15

Salinity in ppt
Pretreatment.       31
Treatment.            65
Recovery.              32

Brood collection
2H13-16
Brood.     1
Gaping.   88
Dead.       0
Closed.    9

Brooders
#         size.     Sick
1.         23.         W

2S9-12
Brood.      6
Gaping     71
Dead.        0
Closed.     14

Brooders
#     size.     Sick
1.     30.        W
2.     32.         W
3.     32         W
4.      41.        W
5.       32.      Grey
6.       21.       W

2N5-8
Brood.      1
Gaping.     82
Dead.        0
Closed.      13

Brooders
#     size.     Sick
1.       28.      W
 

7 3 2014 oyster bay repro check

Shelton, wa

Mid 60s to high 70s cloudy mostly till 2 pm then sunny

Performed the anesthesia sop with insulation on treatment. No ice blocks needed. Found no brooders in North pop but 3 in dabob. Lots still from south sound pop.

Numbers as follow

Temps in c
Pretreatment
Initial.      15
45.            15
1.5.           16

Treatment
Initial.       11
45.             10
1.5.            11
2.25.          11

Recovery
Initial.     16
45.           19
1.5.           22 sun came out. Kept adding water.

Salinity in ppt
Pretreatment.       25
Treatment.            73
Recovery.              26

Brood collection
1N5-8
Brood.    0
Gaping.   49
Dead.      0
Closed.    1

1H1-4
Brood.       3
Gaping.     66
Dead.         4
Closed.      4

Brooders
#          size.      Sick
1.          25.         W
2.          26.          Grey
3.          25.          W

1S13-16
Brood.     9
Gaping.   78
Dead.      0
Closed.    3

Brooders
#       size.        Sick
1.       26.           W
2.       28.           W
3.       26.           W
4.       30.           W
5.       39.           W
6.       25.           W
7.       28.           W
8.       29.           W
9.       25.           W. No larvae collected from 9. Tile oyster.