5 7 2015 Oly Seed DNA check for GBS

Today I checked all the seed DNA isolations using the nanodrop to get concentrations and quality checks done for the Oly GBS project. You can read the results below.

Sample ID	User ID	Date	Time	ng/ul	A260	A280	260/280	260/230	Constant	Cursor Pos.	Cursor abs.	340 raw
HL1	Default	5/7/2015	10:16 AM	39.16	0.979	0.515	1.9	1.7	40	230	0.576	0.016
HL2	Default	5/7/2015	10:17 AM	141.47	3.537	1.749	2.02	2.27	40	230	1.559	0.043
HL3	Default	5/7/2015	10:18 AM	58.05	1.451	0.716	2.03	2.19	40	230	0.664	0.024
HL4	Default	5/7/2015	10:19 AM	119.39	2.985	1.504	1.98	2.27	40	230	1.314	0.064
HL5	Default	5/7/2015	10:20 AM	64.32	1.608	0.805	2	2.2	40	230	0.732	0.053
HL6	Default	5/7/2015	10:21 AM	78.93	1.973	0.976	2.02	2.22	40	230	0.89	0.033
HL7	Default	5/7/2015	10:22 AM	115.35	2.884	1.419	2.03	2.3	40	230	1.251	0.049
HL8	Default	5/7/2015	10:23 AM	11.77	0.235	0.129	1.82	1.54	50	230	0.153	0.055
HL9	Default	5/7/2015	10:25 AM	172.45	3.449	1.726	2	2.31	50	230	1.495	0.043
HL10	Default	5/7/2015	10:26 AM	96.08	1.922	0.966	1.99	2.2	50	230	0.875	0.029
HL11	Default	5/7/2015	10:27 AM	192.3	3.846	1.951	1.97	2.28	50	230	1.689	0.081
HL12	Default	5/7/2015	10:28 AM	58.12	1.162	0.584	1.99	2.15	50	230	0.542	0.047
HL13	Default	5/7/2015	10:29 AM	43.74	0.875	0.451	1.94	2.08	50	230	0.421	0.015
HL14	Default	5/7/2015	10:30 AM	127.92	2.558	1.275	2.01	2.35	50	230	1.09	0.03
HL15	Default	5/7/2015	10:31 AM	28.71	0.574	0.305	1.88	2.01	50	230	0.285	0.015
HL16	Default	5/7/2015	10:32 AM	98.46	1.969	0.978	2.01	2.3	50	230	0.858	0.012
HL17	Default	5/7/2015	10:33 AM	73.78	1.476	0.724	2.04	2.32	50	230	0.635	0.037
HL18	Default	5/7/2015	10:34 AM	66.94	1.339	0.668	2	2.22	50	230	0.604	0.044
HL19	Default	5/7/2015	10:35 AM	287.52	5.75	2.813	2.04	2.34	50	230	2.453	0.019
HL20	Default	5/7/2015	10:36 AM	144.94	2.899	1.412	2.05	2.33	50	230	1.246	0.038
HL21	Default	5/7/2015	10:37 AM	356.18	7.124	3.63	1.96	2.35	50	230	3.026	0.04
HL22	Default	5/7/2015	10:38 AM	222.98	4.46	2.234	2	2.39	50	230	1.869	0.019
HL23	Default	5/7/2015	10:39 AM	393.05	7.861	4.097	1.92	2.33	50	230	3.371	0.035
HL24	Default	5/7/2015	10:40 AM	60.32	1.206	0.62	1.95	2.11	50	230	0.571	0.076
HL25	Default	5/7/2015	10:41 AM	152.2	3.044	1.549	1.96	2.3	50	230	1.321	0.217
HL25	Default	5/7/2015	10:42 AM	142.1	2.842	1.41	2.02	2.67	50	230	1.066	-0.304
HL25	Default	5/7/2015	10:43 AM	128.42	2.568	1.29	1.99	2.29	50	230	1.122	0.097
HL26	Default	5/7/2015	10:44 AM	336.54	6.731	3.345	2.01	2.41	50	230	2.797	-0.006
HL27	Default	5/7/2015	10:45 AM	377.31	7.546	3.813	1.98	2.32	50	230	3.251	0.086
HL28	Default	5/7/2015	10:46 AM	103.32	2.066	1.07	1.93	2.24	50	230	0.921	0.085
HL29	Default	5/7/2015	10:47 AM	74.49	1.49	0.768	1.94	2.2	50	230	0.676	0.047
HL30	Default	5/7/2015	10:48 AM	274.08	5.482	2.804	1.95	2.26	50	230	2.427	0.207
HL31	Default	5/7/2015	10:50 AM	94.38	1.888	0.947	1.99	2.18	50	230	0.866	0.293
HL32	Default	5/7/2015	10:51 AM	269.6	5.392	2.786	1.94	2.16	50	230	2.499	0.3
NF1	Default	5/7/2015	10:53 AM	330.81	6.616	3.396	1.95	2.32	50	230	2.851	0.025
NF1	Default	5/7/2015	10:54 AM	356.37	7.127	3.676	1.94	2.31	50	230	3.09	0.044
NF2	Default	5/7/2015	10:55 AM	210.64	4.213	2.199	1.92	2.35	50	230	1.79	0.043
NF3	Default	5/7/2015	10:56 AM	242.37	4.847	2.514	1.93	2.21	50	230	2.196	0.254
NF4	Default	5/7/2015	10:57 AM	207.32	4.146	2.201	1.88	2	50	230	2.077	2.737
NF5	Default	5/7/2015	10:58 AM	323.45	6.469	3.368	1.92	2.11	50	230	3.066	0.363
NF6	Default	5/7/2015	10:59 AM	317.97	6.359	3.327	1.91	2.35	50	230	2.711	0.065
NF6	Default	5/7/2015	11:00 AM	270.17	5.403	2.794	1.93	2.38	50	230	2.271	0.077
NF7	Default	5/7/2015	11:01 AM	191.55	3.831	1.985	1.93	2.27	50	230	1.686	0.07
NF8	Default	5/7/2015	11:02 AM	361.84	7.237	3.677	1.97	2.33	50	230	3.102	0.04
NF9	Default	5/7/2015	11:03 AM	166.86	3.337	1.677	1.99	2.31	50	230	1.447	0.074
NF10	Default	5/7/2015	11:05 AM	124.73	2.495	1.255	1.99	2.22	50	230	1.125	0.135
NF11	Default	5/7/2015	11:06 AM	447.26	8.945	4.552	1.97	2.25	50	230	3.982	0.394
NF12	Default	5/7/2015	11:07 AM	93.36	1.867	0.963	1.94	2.08	50	230	0.896	0.138
NF13	Default	5/7/2015	11:08 AM	277	5.54	2.847	1.95	2.18	50	230	2.547	0.231
NF14	Default	5/7/2015	11:09 AM	298.5	5.97	2.983	2	2.32	50	230	2.576	0.053
NF15	Default	5/7/2015	11:10 AM	155.67	3.113	1.569	1.98	2.3	50	230	1.352	0.051
NF16	Default	5/7/2015	11:11 AM	135.04	2.701	1.396	1.94	2.15	50	230	1.258	0.139
NF17	Default	5/7/2015	11:13 AM	52.7	1.054	0.559	1.88	2.14	50	230	0.492	0.038
NF18	Default	5/7/2015	11:14 AM	292.28	5.846	2.973	1.97	2.21	50	230	2.646	0.311
NF19	Default	5/7/2015	11:15 AM	90.2	1.804	0.934	1.93	2.25	50	230	0.801	0.049
NF20	Default	5/7/2015	11:16 AM	374.23	7.485	3.772	1.98	2.36	50	230	3.176	0.034
NF20	Default	5/7/2015	11:17 AM	876.31	17.526	8.649	2.03	2.24	50	230	7.826	0.316
NF21	Default	5/7/2015	11:18 AM	233.53	4.671	2.416	1.93	2.37	50	230	1.971	0.057
NF22	Default	5/7/2015	11:19 AM	86.59	1.732	0.893	1.94	2.35	50	230	0.736	0.018
NF23	Default	5/7/2015	11:20 AM	204.91	4.098	2.067	1.98	2.35	50	230	1.741	0.034
NF24	Default	5/7/2015	11:21 AM	231.38	4.628	2.391	1.94	2.34	50	230	1.981	0.069
NF25	Default	5/7/2015	11:22 AM	126.3	2.526	1.384	1.82	1.59	50	230	1.593	0.863
NF26	Default	5/7/2015	11:23 AM	159.12	3.182	1.597	1.99	2.31	50	230	1.378	0.103
NF27	Default	5/7/2015	11:25 AM	101.21	2.024	1.058	1.91	2.33	50	230	0.869	0.035
NF28	Default	5/7/2015	11:26 AM	128.03	2.561	1.279	2	2.33	50	230	1.101	0.046
NF29	Default	5/7/2015	11:27 AM	176.44	3.529	1.786	1.98	2.33	50	230	1.515	0.038
NF30	Default	5/7/2015	11:28 AM	187.06	3.741	1.895	1.97	2.31	50	230	1.62	0.061
NF31	Default	5/7/2015	11:29 AM	117.53	2.351	1.179	1.99	2.27	50	230	1.036	0.082
NF32	Default	5/7/2015	11:30 AM	272.95	5.459	2.808	1.94	2.28	50	230	2.394	-0.407
SN1	Default	5/7/2015	11:36 AM	107.82	2.156	1.096	1.97	2.16	50	230	0.998	0.099
SN2	Default	5/7/2015	11:37 AM	278.11	5.562	2.803	1.98	2.26	50	230	2.464	0.124
SN3	Default	5/7/2015	11:38 AM	122.94	2.459	1.255	1.96	2.27	50	230	1.083	0.028
SN4	Default	5/7/2015	11:39 AM	225.29	4.506	2.236	2.01	2.31	50	230	1.949	0.706
SN5	Default	5/7/2015	11:41 AM	89.6	1.792	0.914	1.96	2.21	50	230	0.811	0.056
SN6	Default	5/7/2015	11:42 AM	79.5	1.59	0.808	1.97	2.23	50	230	0.712	0.044
SN7	Default	5/7/2015	11:43 AM	107.8	2.156	1.117	1.93	2.2	50	230	0.978	0.086
SN8	Default	5/7/2015	11:44 AM	212.99	4.26	2.185	1.95	2.25	50	230	1.89	0.417
SN9	Default	5/7/2015	11:45 AM	115.94	2.319	1.206	1.92	2.29	50	230	1.01	0.094
SN10	Default	5/7/2015	11:46 AM	118.82	2.376	1.221	1.95	2.21	50	230	1.074	0.085
SN11	Default	5/7/2015	11:47 AM	267.08	5.342	2.724	1.96	2.32	50	230	2.298	0.105
SN12	Default	5/7/2015	11:49 AM	127.94	2.559	1.317	1.94	2.32	50	230	1.103	0.071
SN13	Default	5/7/2015	11:50 AM	317.61	6.352	3.194	1.99	2.32	50	230	2.74	0.11
SN14	Default	5/7/2015	11:51 AM	146.97	2.939	1.498	1.96	2.23	50	230	1.316	0.08
SN15	Default	5/7/2015	11:52 AM	164.33	3.287	1.665	1.97	2.24	50	230	1.469	0.099
SN16	Default	5/7/2015	11:53 AM	77.75	1.555	0.821	1.89	1.87	50	230	0.833	0.312
SN17	Default	5/7/2015	11:54 AM	125.5	2.51	1.293	1.94	2.24	50	230	1.119	0.025
SN18	Default	5/7/2015	11:56 AM	170.94	3.419	1.748	1.96	2.35	50	230	1.456	0.023
SN19	Default	5/7/2015	11:58 AM	74.9	1.498	0.786	1.91	2.07	50	230	0.724	0.054
SN20	Default	5/7/2015	11:59 AM	104.44	2.089	1.078	1.94	2.33	50	230	0.895	0.026
SN21	Default	5/7/2015	12:00 PM	83.42	1.668	0.857	1.95	2.18	50	230	0.765	0.084
SN22	Default	5/7/2015	12:01 PM	243.91	4.878	2.54	1.92	2.05	50	230	2.381	0.656
SN23	Default	5/7/2015	12:02 PM	365.7	7.314	3.644	2.01	2.31	50	230	3.169	0.07
SN24	Default	5/7/2015	12:03 PM	152.83	3.057	1.588	1.92	2.31	50	230	1.323	0.044
SN25	Default	5/7/2015	12:04 PM	160.41	3.208	1.617	1.98	2.35	50	230	1.363	0.038
SN26	Default	5/7/2015	12:06 PM	71.78	1.436	0.753	1.91	2.24	50	230	0.642	0.053
SN27	Default	5/7/2015	12:07 PM	244.35	4.887	2.454	1.99	2.35	50	230	2.076	0.027
SN28	Default	5/7/2015	12:08 PM	153.82	3.076	1.561	1.97	2.33	50	230	1.322	0.045
SN29	Default	5/7/2015	12:09 PM	192.01	3.84	1.975	1.94	2.24	50	230	1.711	0.108
SN30	Default	5/7/2015	12:10 PM	293	5.86	2.947	1.99	2.33	50	230	2.519	0.051
SN31	Default	5/7/2015	12:11 PM	321.32	6.426	3.337	1.93	2.32	50	230	2.765	0.085
SN32	Default	5/7/2015	12:12 PM	45.65	0.913	0.477	1.91	1.89	50	230	0.483	0.104

You can also see the raw nanodrop file here and the raw tab delimited table here.

**Update**
After reviewing the spec results from the Plate and the nanodrop results here. I have chosen the 40 samples to be sent to GBS. The samples from the Plate need to be renamed to have a P in front of the sample ID so that I can tell they were from the plate.

Samples from the Mollusk EZNA Kit in individual 1.5 ml tubes:

Sample ID	Number	Date	Time	ng/ul	A260	A280	260/280	260/230
HL10	1	5/7/2015	10:26 AM	96.08	1.922	0.966	1.99	2.2
HL11	2	5/7/2015	10:27 AM	192.3	3.846	1.951	1.97	2.28
HL12	3	5/7/2015	10:28 AM	58.12	1.162	0.584	1.99	2.15
HL14	4	5/7/2015	10:30 AM	127.92	2.558	1.275	2.01	2.35
HL16	5	5/7/2015	10:32 AM	98.46	1.969	0.978	2.01	2.3
HL17	6	5/7/2015	10:33 AM	73.78	1.476	0.724	2.04	2.32
HL18	7	5/7/2015	10:34 AM	66.94	1.339	0.668	2	2.22
HL19	8	5/7/2015	10:35 AM	287.52	5.75	2.813	2.04	2.34
HL2	9	5/7/2015	10:17 AM	141.47	3.537	1.749	2.02	2.27
HL20	10	5/7/2015	10:36 AM	144.94	2.899	1.412	2.05	2.33
HL21	11	5/7/2015	10:37 AM	356.18	7.124	3.63	1.96	2.35
HL22	12	5/7/2015	10:38 AM	222.98	4.46	2.234	2	2.39
HL23	13	5/7/2015	10:39 AM	393.05	7.861	4.097	1.92	2.33
HL24	14	5/7/2015	10:40 AM	60.32	1.206	0.62	1.95	2.11
HL25	15	5/7/2015	10:41 AM	152.2	3.044	1.549	1.96	2.3
HL25	16	5/7/2015	10:42 AM	142.1	2.842	1.41	2.02	2.67
HL25	17	5/7/2015	10:43 AM	128.42	2.568	1.29	1.99	2.29
HL26	18	5/7/2015	10:44 AM	336.54	6.731	3.345	2.01	2.41
HL27	19	5/7/2015	10:45 AM	377.31	7.546	3.813	1.98	2.32
HL28	20	5/7/2015	10:46 AM	103.32	2.066	1.07	1.93	2.24
HL29	21	5/7/2015	10:47 AM	74.49	1.49	0.768	1.94	2.2
HL3	22	5/7/2015	10:18 AM	58.05	1.451	0.716	2.03	2.19
HL30	23	5/7/2015	10:48 AM	274.08	5.482	2.804	1.95	2.26
HL31	24	5/7/2015	10:50 AM	94.38	1.888	0.947	1.99	2.18
HL32	25	5/7/2015	10:51 AM	269.6	5.392	2.786	1.94	2.16
HL4	26	5/7/2015	10:19 AM	119.39	2.985	1.504	1.98	2.27
HL5	27	5/7/2015	10:20 AM	64.32	1.608	0.805	2	2.2
HL6	28	5/7/2015	10:21 AM	78.93	1.973	0.976	2.02	2.22
HL7	29	5/7/2015	10:22 AM	115.35	2.884	1.419	2.03	2.3
HL9	30	5/7/2015	10:25 AM	172.45	3.449	1.726	2	2.31
NF1	1	5/7/2015	10:53 AM	330.81	6.616	3.396	1.95	2.32
NF10	2	5/7/2015	11:05 AM	124.73	2.495	1.255	1.99	2.22
NF11	3	5/7/2015	11:06 AM	447.26	8.945	4.552	1.97	2.25
NF12	4	5/7/2015	11:07 AM	93.36	1.867	0.963	1.94	2.08
NF13	5	5/7/2015	11:08 AM	277	5.54	2.847	1.95	2.18
NF14	6	5/7/2015	11:09 AM	298.5	5.97	2.983	2	2.32
NF15	7	5/7/2015	11:10 AM	155.67	3.113	1.569	1.98	2.3
NF16	8	5/7/2015	11:11 AM	135.04	2.701	1.396	1.94	2.15
NF18	9	5/7/2015	11:14 AM	292.28	5.846	2.973	1.97	2.21
NF19	10	5/7/2015	11:15 AM	90.2	1.804	0.934	1.93	2.25
NF2	11	5/7/2015	10:55 AM	210.64	4.213	2.199	1.92	2.35
NF20	12	5/7/2015	11:16 AM	374.23	7.485	3.772	1.98	2.36
NF21	13	5/7/2015	11:18 AM	233.53	4.671	2.416	1.93	2.37
NF22	14	5/7/2015	11:19 AM	86.59	1.732	0.893	1.94	2.35
NF23	15	5/7/2015	11:20 AM	204.91	4.098	2.067	1.98	2.35
NF24	16	5/7/2015	11:21 AM	231.38	4.628	2.391	1.94	2.34
NF25	17	5/7/2015	11:22 AM	126.3	2.526	1.384	1.82	1.59
NF26	18	5/7/2015	11:23 AM	159.12	3.182	1.597	1.99	2.31
NF27	19	5/7/2015	11:25 AM	101.21	2.024	1.058	1.91	2.33
NF28	20	5/7/2015	11:26 AM	128.03	2.561	1.279	2	2.33
NF29	21	5/7/2015	11:27 AM	176.44	3.529	1.786	1.98	2.33
NF3	22	5/7/2015	10:56 AM	242.37	4.847	2.514	1.93	2.21
NF30	23	5/7/2015	11:28 AM	187.06	3.741	1.895	1.97	2.31
NF31	24	5/7/2015	11:29 AM	117.53	2.351	1.179	1.99	2.27
NF32	25	5/7/2015	11:30 AM	272.95	5.459	2.808	1.94	2.28
NF4	26	5/7/2015	10:57 AM	207.32	4.146	2.201	1.88	2
NF5	27	5/7/2015	10:58 AM	323.45	6.469	3.368	1.92	2.11
NF6	28	5/7/2015	10:59 AM	317.97	6.359	3.327	1.91	2.35
NF6	29	5/7/2015	11:00 AM	270.17	5.403	2.794	1.93	2.38
NF7	30	5/7/2015	11:01 AM	191.55	3.831	1.985	1.93	2.27
NF8	31	5/7/2015	11:02 AM	361.84	7.237	3.677	1.97	2.33
NF9	32	5/7/2015	11:03 AM	166.86	3.337	1.677	1.99	2.31
SN1	1	5/7/2015	11:36 AM	107.82	2.156	1.096	1.97	2.16
SN10	2	5/7/2015	11:46 AM	118.82	2.376	1.221	1.95	2.21
SN11	3	5/7/2015	11:47 AM	267.08	5.342	2.724	1.96	2.32
SN12	4	5/7/2015	11:49 AM	127.94	2.559	1.317	1.94	2.32
SN13	5	5/7/2015	11:50 AM	317.61	6.352	3.194	1.99	2.32
SN14	6	5/7/2015	11:51 AM	146.97	2.939	1.498	1.96	2.23
SN15	7	5/7/2015	11:52 AM	164.33	3.287	1.665	1.97	2.24
SN17	8	5/7/2015	11:54 AM	125.5	2.51	1.293	1.94	2.24
SN18	9	5/7/2015	11:56 AM	170.94	3.419	1.748	1.96	2.35
SN2	10	5/7/2015	11:37 AM	278.11	5.562	2.803	1.98	2.26
SN20	11	5/7/2015	11:59 AM	104.44	2.089	1.078	1.94	2.33
SN21	12	5/7/2015	12:00 PM	83.42	1.668	0.857	1.95	2.18
SN22	13	5/7/2015	12:01 PM	243.91	4.878	2.54	1.92	2.05
SN23	14	5/7/2015	12:02 PM	365.7	7.314	3.644	2.01	2.31
SN24	15	5/7/2015	12:03 PM	152.83	3.057	1.588	1.92	2.31
SN25	16	5/7/2015	12:04 PM	160.41	3.208	1.617	1.98	2.35
SN27	17	5/7/2015	12:07 PM	244.35	4.887	2.454	1.99	2.35
SN28	18	5/7/2015	12:08 PM	153.82	3.076	1.561	1.97	2.33
SN29	19	5/7/2015	12:09 PM	192.01	3.84	1.975	1.94	2.24
SN3	20	5/7/2015	11:38 AM	122.94	2.459	1.255	1.96	2.27
SN30	21	5/7/2015	12:10 PM	293	5.86	2.947	1.99	2.33
SN31	22	5/7/2015	12:11 PM	321.32	6.426	3.337	1.93	2.32
SN4	23	5/7/2015	11:39 AM	225.29	4.506	2.236	2.01	2.31
SN5	24	5/7/2015	11:41 AM	89.6	1.792	0.914	1.96	2.21
SN7	25	5/7/2015	11:43 AM	107.8	2.156	1.117	1.93	2.2
SN8	26	5/7/2015	11:44 AM	212.99	4.26	2.185	1.95	2.25
SN9	27	5/7/2015	11:45 AM	115.94	2.319	1.206	1.92	2.29
SN6	28	5/7/2015	11:42 AM	79.5	1.59	0.808	1.97	2.23
SN16	29	5/7/2015	11:53 AM	77.75	1.555	0.821	1.89	1.87

Samples from the DNEasy Plate:

Sample	ng/ul	plate designation	number
PHL04	76.40	D1	1
PHL15	71.58	G2	2
PHL18	86.78	B3	3
PHL21	80.37	E3	4
PHL24	73.51	H3	5
PHL26	72.73	B4	6
PHL27	103.28	C4	7
PHL31	79.00	G4	8
PHL14	69.76	F2	9
PHL20	59.87	D3	10
PNF02	77.88	B5	1
PNF07	83.26	G5	2
PNF19	91.79	C7	3
PNF22	73.80	F7	4
PNF29	76.51	E8	5
PNF32	79.92	H8	6
PNF04	68.87	D5	7
PNF25	67.80	A8	8
PSN03	83.00	C9	1
PSN07	97.94	G9	2
PSN09	79.18	A10	3
PSN12	80.85	D10	4
PSN13	78.33	E10	5
PSN22	77.95	F11	6
PSN23	92.72	G11	7
PSN27	83.17	C12	8
PSN28	90.17	C13	9
PSN29	82.61	C14	10
PSN30	85.23	C15	11