Jake Heare Research Central: 5-14 to 15-16 2014 Reproduction Work Up

5-14-2014

Manchester WA

Participants: Brent Vadopalas and Jake Heare

We retrieved the next set of trays in the dosing sequence and allowed each tray to dessicate for 45 minutes. Trays were then treated in a 10 gallon bath of 50% sea water to 50% freshwater mixed with 7 lbs of epsom salt. The tray was treated for 45 minutes, at which point it was removed and gaping animals were examined for signs of brooding. After examination the trays were then placed into a recovery tub with 100% sea water until the last tray examined had be in the recovery tub for 45 minutes. After each treatment the treatment water was replaced with fresh treatment to reduce temperature flux. Then trays were rebuilt into a stack and hung off the dock.

We counted the number of gaping animals and brooders for each tray.

They are as follows:

4H1-4

brooders 0

Gaping 25

Closed 72
% Open 25.8%

4S9-12

Brooders 0

Gaping 43

Closed 55
% Open 43.9%

4N9-12

Brooders 0

Gaping 31

Closed 28
% Open 52.5%

5-15-2014

Oyster Bay WA

Participants: Katie Jackson and Jake Heare

We followed a procedure similar to that at Manchester. The difference being that the treatment water was not replaced after each treatment. We also counted the dead in each tray.

1H1-4

Brooders 0

Gaping 49

Dead 7

Closed 33
% Open 59.8%

1N5-8

Brooders 0

Gaping 46

Dead 7

Closed 14
% Open 76.7%

1S13-16

Brooders 0