5 30 2014 fidalgo repro check

Anacortes wa

Mid 60s to mid 70s

Performed standard reproduction check using the anesthesia sop. Found no brooders. Also surface water Temps at 3 pm were only 9C.

Temps
Pretreatment
Initial 10
45 min. 12
1.5 hrs   16

Treatment
Initial. 9
45 min.  13
1.5 hrs.  16
2.25 hrs.  19

Recovery
Initial.  9
45 min. 13
1.5 hrs.  16

Salinity
Seawater.  30 ppt
Treatment.  65 ppt

Brood
2S5-8
Brood.   0
Gaping.  77
Dead.      1

2N9-12
Brood.   0
Gaping.  46
Dead.     0

2H1-4
Brood.  0
Gaping. 50
Dead.   1

Also forgot to dessicate 2N tray. Placed it straight in treatment from bath. Still got ok reaction from it.