Oyster Bay 9/19/2014
Fidalgo Bay 10/17/2014
Sample Layout in 96 Well Plate
|A||1N1-4 1||1N1-4 2||1N1-4 3||1N1-4 4||1N1-4 5||1N1-4 6||1N1-4 7||1N1-4 8||1N1-4 11||1N1-4 10||1H1-4 1||1H1-4 2|
|B||1H1-4 3||1H1-4 4||1H1-4 5||1H1-4 6||1H1-4 7||1H1-4 8||1H1-4 9||1H1-4 10||1S1-4 1||1S1-4 2||1S1-4 3||1S1-4 4|
|C||1S1-4 5||1S1-4 6||1S1-4 7||1S1-4 8||1S1-4 9||1S1-4 10||2N1-4 1||2N1-4 2||2N1-4 3||2N1-4 4||2N1-4 5||2N1-4 6|
|D||2N1-4 7||2N1-4 8||2N1-4 9||2N1-4 10||2H1-4 1||2H1-4 2||2H1-4 3||2H1-4 4||2H1-4 5||2H1-4 6||2H1-4 7||2H1-4 8|
|E||2H1-4 9||2H1-4 10||2S1-4 1||2S1-4 2||2S1-4 3||2S1-4 4||2S1-4 5||2S1-4 6||2S1-4 7||2S1-4 8||2S1-4 9||2S1-4 10|
|F||4N1-4 1||4N1-4 2||4N1-4 3||4N1-4 4||4N1-4 5||4N1-4 6||4N1-4 7||4N1-4 8||4N1-4 9||4N1-4 10||4H1-4 1||4H1-4 2|
|G||4H1-4 3||4H1-4 4||4H1-4 5||4H1-4 6||4H1-4 7||4H1-4 8||4H1-4 9||4H1-4 10||4S1-4 1||4S1-4 2||4S1-4 3||4S1-4 4|
|H||4S1-4 5||4S1-4 6||4S1-4 7||4S1-4 8||4S1-4 9||4S1-4 10||Control||Control||Control||Control||Control||Control|
I carefully placed each sample in the corresponding microcollection tube by removing the tissue from the original 1.5 ml sample tubes with flame sterilized forceps and gently sliding it into the collection tube.
Then I created the working buffer by 2 ml proteinase K to 17.5 ml ATL buffer from the Qiagen kit into a 50 ml falcon tube. Then briefly vortexed the mixture and poured it into a channel pipetter liquid vessel.
Using the 12 Channel Pipetter I pipetted 200 ul of the working buffer into each tube.
I mixed the solution using inversion and then checked to see if all samples were submerged in the working lysis buffer.
For samples that were not in the buffer I used a clean 1000 ul pipette tip to gently slide the sample into the buffer.
All samples were fully sumberged in the mixture and are now incubating at 56 C in FTR 228.