Manchester WA
Temps Mid 60's to High 70s
Sunny
Participants: Joelle Blais and Jake Heare
Used the Anesthesia SOP to check for brooding larvae. I modified this per Steven and Brent's suggestions to temperature control via adding ice blocks to the solution as well as insulate the tub with 2 inch styrofoam insulation with foil top and bottom. Much to my surprise not only did the insulation work, kept water within 1 degree of ambient with out ice blocks, it actually lowered the temps of the treatment when ice blocks were added (2 degrees below ambient). I believe this insulation and ice blocks method will be the way to go from here on out. Even on the warmest days I see it insulating the tubs very well.
Numbers as follow:
Temps in C
Pretreatment
Initial 9
45 min 15
1.5 hrs 19
Treatment
Initial 11
45 min 11
1.5 hrs 10
2.25 hrs 8
Recovery
Initial 11
45 mins 13
1.5 hrs 15
Salinity in ppt
Pretreatment 25
Treatment 65
Recovery 25
Brood Collection
4H1-4
Brood 0
Gapin 63
Dead 2
Closed 31
4S9-12
Brood 0
Gaping 49
Dead 12
Closed 37
4N9-12
Brood 0
Gaping 41
Dead 8
Closed 12
One male in the 4H group had sperm within its shell. I assume these animals are attempting to spawn though water temps seem to be below expectation.
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