8 15 14 fidalgo repro check

Anacortes wa

High 50s to low 70s mostly cloudy light rain early dry later

Performed the anesthesia sop with insulation on treatment tub. weather allowed to go without ice blocks or tarp. Found no brooders. Thought that was weird. Checked previous entries and realized I treated the same stack twice in a row due to an error I made last week. Not sure if it really signifies the end of spawning or if I'm really efficient at collecting larvae.

Numbers as follow

Temps in c
Pretreatment
Initial.   12
45.       12
1.5.       13

Treatment
Initial.    12
45.        12
1.5.        12
2.25.      13

Recovery
Initial.     12
45.         13
1.5.         14

Salinity in ppt
Pretreatment.   25
Treatment.       63
Recovery.        24

Brood collection

2N13-16
Brood.    0
Gaping.   78
Dead.      0
Closed.    25

2H9-12
Brood.    0
Gaping.   83
Dead.      0
Closed.    3

2S1-4
Brood.   0
Gaping.  88
Dead.     0
Closed.   6

Pics have closed on same tile as tube label and calipers. Gapers on all other tiles.