8 8 2014 fidalgo repro check

Anacortes wa

Mid 60s to high 70s sunny

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Treatment was effective as normal so it seemed to be a fluke the last couple of weeks. Also had pretty equal brooding across all populations. Finally the northern population had the most number of brooders. I think we should check these animals again around the full moon in September to see if they are still spawning.

Numbers as follow :

Temps in c
Pretreatment
Initial.   11
45.      13
1.5.       15

Treatment
Initial.     13
45.        14
1.5.         13
2.25.       14

Recovery
Initial.     11
45.        12
1.5.         15

Salinity in ppt
Pretreatment.       27
Treatment.           56
Recovery.            28

Brood collection
2S1-4
Brood.      2
Gaping.     70
Dead.        0
Closed.      22

Brooders
#   size.  Sick
1.     32.    Grey
2.     30.    W

2H9-12
Brood.       3
Gaping.      67
Dead.         0
Closed.      15

Brooders
#     size.     Sick
1.      30.       W
2.     29.        W
3.      20.      W

2N13-16
Brood.     5
Gaping.    89
Dead.       0
Closed.    10

Brooders
#   size.   Sick
1.     19.      W
2.    34.      W
3.    35.      W
4.    32.      W
5.    29.      W

Pics have brooders between Tube and calipers. Closed on same tile. Gaping on all other tiles.